The antibacterial activity of honey obtained from different locations in Enugu North and South(Nsukka and Ugwuaji) Nigeria on Streptococcus pyogenes,, Escherichia coli and Staphylococcus aureus isolated from wound swabs collected from University of Nigeria Teaching Hospital (UNTH) were studied. Reviews on the effects of honey in medicine and its properties was further studied, because as an antimicrobial agent honey may have the potential for treating a variety of ailments. The Antibacterial properties of honey are the result of the low water activity causing osmosis, hydrogen peroxide effect, and high acidity content . Gram test and biochemical tests were carried out on the cultures. The method employed for the sensitivity test was the agar well diffusion assay, which involved the determination of antibacterial activity by measuring the zones of inhibition produced by honey, measured by using a transparent meter rule. The results of this study revealed antibacterial activity of both the honey samples used on the test organisms as the concentration of honey was increased, high antibacterial activity was attained . Honey from Nsukka was found to be more potent on the test organisms by producing the largest zone of inhibition on Staph.aureus and E.coli at all dilution concentrations and inhibited moderately on Streptococcus pyogenes.