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A total of 500 samples (292 stool samples from human (diarrhea and non diarrhea) patients and 208 samples from different transmission vehicles such as ice cream, sachet water, stream water, borehole water, rain water, well water and beef suya) were screened from the six health zones of the state. The isolation methods adopted were cold enrichment method using phosphate buffered saline (pH 7.2) and treatment of one loopful of the enriched cell with an alkaline solution (0.5% KOH in 0.5% NaCl) prior to subculture onto selective solid culture medium [Cefuslodin Irgasan Novobiocin (CIN) agar]. Out of 500 samples screened, 112 samples were Yersinia enterocolitica positive; 75 from human and 37 from transmission vehicles. The incidence was highest among those under 1-10 years 35(36.1%). There was no record of Yersinia enterocolitica on patients between the ages of 41-50 and 71-80. The incidence was highest in beef Suya with 63.3% and lowest on well water with 4.4% from transmission vehicles. No Yersinia enterocolitica was isolated from sachet water, rain water and borehole water. The incidence of Yersinia enterocolitica was dependent on epidemiological factors like age, occupation, safe toilet facility and nature of specimen. Yersinia enterocolitica isolates were all susceptible to Ciprofloxacin, Ofloxacin, Gentamicin and tetracycline. Three isolates were susceptible to Augumentin. All the isolates were resistant to Cotrimazole and Amoxicillin. No Yersinia enterocolitica isolate from transmission vehicles was pathogenic to mice. Fifty isolates from human samples were pathogenic to mice. Isolates recovered from experimentally infected mice had the same characteristics as the initial isolates introduced into the mice.

Bank: GTBank
Account Name: Prince Orafu
Account Number: 0109980866

Bank: Diamond Bank
Account Name: Prince Orafu
Account Number: 0065070861

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