Yersinia enterocolitica is a primary cause of zoonotic gasteroenteritis in animals and man. Not much information exists on the incidence of Yersinia enterocolitica in domestic animals in Nigeria especially Enugu State. A total of five hundred (500) faecal samples were randomly collected from various domestic animals in ten (10) different farms within Enugu State for the analysis. The domestic animals used were pigs, goats, sheep, cattle, horses, donkeys, chicken and turkeys. After the various faecal samples were collected, they were incubated in PBS (phosphate buffered saline) at 4°C for 3 weeks after which the organism was isolated using CIN agar ( Cefsulodin-Irgasan-Novobiocin agar). One hundred and forty five (145) positive isolates were isolated out of which 78 (seventy eight) were identified as pathogenic strains of Yersinia enterocolitica following pathogenicity testing in mice. The organisms were sensitive to Gentamicin, Ofloxacin, Siprosan and Cotrimazole. It was observed that the incidence of Yersinia enterocolitica in domestic animals was dependent on epidemiological factors such as poor cleaning of the farms, inadequate washing of food/water troughs, type of feed given to the animals, animal specie, age of the animal and system of husbandry. Adequate farm hygiene is encouraged to control the spread/occurrence of this organism.